by Thomas Spieker | Dec 9, 2015 | Tips&Tricks
Fluorescence is a great technique for microscopy. It provides amazing contrast and it allows us to selectively view the objects that interest us without being obscured by surrounding structures. The one big drawback it has over brightfield techniques is bleaching....
by Thomas Spieker | Nov 23, 2015 | Tips&Tricks
When I first started out in microscopy, the only way to localize a protein inside a cell was using antibodies. That meant fixing your specimen, going through a complicated series of incubations with blocking agents, primary, and secondary antibodies before you could...
